Microscope Control Software

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|[[Image:Leica.jpg|thumb|320px|Screenshot of software controlling a microscope developed with mimas.]]
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|[[Image:Leica.jpg|thumb|160px|Screenshot of software controlling a microscope developed with mimas.]]||
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[[Image:Syringe.png|thumb|160px|Image of syringe chip, captured with the software]]
 
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|[[Image:Syringe.png|thumb|320px|Image of syringe chip, captured with the software]]
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|[[Image:Cells1.jpg|thumb|160px|Microscope image (FOV width 1 mm) of eucaryote [http://vision.eng.shu.ac.uk/jan/cells1.avi (4.8MB video)]]]||
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[[Image:Cells2.jpg|thumb|160px|Microscope image (FOV width 500 um) [http://vision.eng.shu.ac.uk/jan/cells2.avi (6.7MB video)]]]
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|[[Image:Cells3.jpg|thumb|160px|Microscope image (FOV width 100 um) of eucaryote [http://vision.eng.shu.ac.uk/jan/cells3.avi (12.6MB video)]]]||
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[[Image:Cells4.jpg|thumb|160px|Microscope image (FOV width 500 um) of eucaryote [http://vision.eng.shu.ac.uk/jan/cells4.avi (934kB video)]]]
 
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The software uses OpenGL to display the camera image with a high framerate. The software already has the following capabilities:
 
The software uses OpenGL to display the camera image with a high framerate. The software already has the following capabilities:
 
* Move object table with mouse-dragging
 
* Move object table with mouse-dragging
* Camera-display with digital zoom
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* 640x480 Camera-display with digital zoom
 
* Display and capture videos:
 
* Display and capture videos:
 
** Colour images
 
** Colour images

Revision as of 16:16, 15 October 2005

Screenshot of software controlling a microscope developed with mimas.
Image of syringe chip, captured with the software
Microscope image (FOV width 1 mm) of eucaryote (4.8MB video)
Microscope image (FOV width 500 um) (6.7MB video)
Microscope image (FOV width 100 um) of eucaryote (12.6MB video)
Microscope image (FOV width 500 um) of eucaryote (934kB video)

At the MMVL a Linux software for controlling a Leica DM LAM microscope with a Basler A302fc firewire camera has been developed.

The software uses OpenGL to display the camera image with a high framerate. The software already has the following capabilities:

  • Move object table with mouse-dragging
  • 640x480 Camera-display with digital zoom
  • Display and capture videos:
    • Colour images
    • Graylevel images
    • Edge images (thresholded Sobel)
  • Capture focus stacks

The implementation took maybe 15 days. Before being able to develop the application itself, the Mimas-library had to be enhanced with firewire digital camera input, the existing libserial-library had to be enhanced with timing functionality and the required part of the serial communication with the Leica DM LAM microscope had to be implemented under Linux.

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