Pipette Reconstruction

Pipette imaged by inverted microscope

Image with pipette out of focus

Reconstruction of pipette

Reconstruction of pipette

Image with extended depth of field

The micro-pipette is tilted at an unknown angle. A sequence of images is taken by varying the height of the microscope-table. The resulting focus stack can be used for reconstruction by maximising the values of a fast 1-D Haynes Jain filter.